Scanning Calorimetry Studies of the Micelle-Vesicle Transition In Phospholipids

磷脂中胶束-囊泡转变的扫描量热研究

• 批准号: 8820849
• 负责人: Charles Spink
• 金额: $ 8.76万
• 依托单位: SUNY College at Cortland
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820849&HistoricalAwards=false
• 关键词: Scanning; Calorimetry; Studies; Micelle; Vesicle

Mixtures of phospholipid and detergents are known to undergo transformation between micelle and vesicle forms under proper conditions. The transformation process occurs within a narrow range of compositions of detergent and phospholipid and seems to involve intermediate structures which are quite different from either the vesicles or micelles which exist at the two extreme boundaries of the transition region. Little is known about the characteristics of the transitions in the transition region and the properties of the transitions forms. This project involves the study of several detergent- phospholipid systems by differential scanning calorimetry at compositions particularly close to the transition region between micelles and vesicles. Lipids in aqueous mixtures of phospholipids and detergent are thought to exist in several different structural forms dependent upon the composition and conditions in solution. At low mole ratios of detergent to lipid unilamellar vesicles form, while at high ratios micellar structures prevail. The transition between these limiting structures is a fundamental problem that has implications for several important areas relating to the physical and chemical behavior of phospholipids. The results of this project could be significant for a better understanding of membrane reconstitution methods, vesicular drug delivery methods and the mechanisms of lipid transport in biliary mixtures of bile salts and phospholipids.

已知磷脂和洗涤剂的混合物在适当条件下会在胶束和囊泡形式之间发生转化。 转化过程发生在清洁剂和磷脂的狭窄范围内，似乎涉及中间结构，这些结构与过渡区域两个极端边界存在的囊泡或胶束截然不同。 关于过渡区域中过渡的特征以及过渡形式的特性知之甚少。 该项目涉及对几种洗涤剂 - 磷脂系统的研究，这是通过在胶束和小囊泡之间的过渡区域的组成量差扫描量热法进行的。 人们认为，磷脂和洗涤剂水性混合物中的脂质被认为存在于几种不同的结构形式中，取决于溶液中的组成和条件。 在低摩尔比脂质单层囊泡的低摩尔比下形成，而在高比率下，胶束结构占了上风。 这些限制结构之间的过渡是一个基本问题，对与磷脂的物理和化学行为有关的几个重要领域具有影响。 该项目的结果对于更好地理解膜重建方法，囊泡药物递送方法以及胆汁盐和磷脂胆汁混合物中脂质转运的机制可能很重要。