Mechanisms of Virus-Mediated Translational Inhibition

病毒介导的翻译抑制机制

• 批准号: 8816918
• 负责人: Victor Chinchar
• 金额: $ 10万
• 依托单位: University of Mississippi Medical Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8816918&HistoricalAwards=false
• 关键词: Mechanisms; Virus; Mediated; Translational; Inhibition

Attempts are being made to determine the mechanism by which frog virus 3 selectively blocks host cell translation. To accomplish this it is planned to identify and isolate the FV3 virion-associated translational inhibitor, to determine the mechanism of FV3 mediated translational shut-off by identifying where in initiation the translational defect occurs, and to determine whether the 5' untranslated region of viral messages controls their translational efficiency. During the past twenty years, the sequence of events and specific components involved in eukaryotes have been determined and the importance of translation control in gene expression has gained general accpetance. However, despite advances in both areas, there remain significant gaps. For example the precise roles for several eukaryotic initiation factors and function(s) of their various subunits is not known. In light of this, it should be apparent that model systems, of which FV3 is one, will be most important in advancing our understanding of translation and translational control. Dr. Chinchar is well qualified to carry out this research.

人们正在尝试确定青蛙病毒3选择性阻断宿主细胞翻译的机制。 为了实现这一目标，计划鉴定和分离 FV3 病毒体相关的翻译抑制剂，通过鉴定翻译缺陷发生的起始位置来确定 FV3 介导的翻译关闭机制，并确定病毒的 5' 非翻译区是否存在。消息控制着它们的翻译效率。 在过去的二十年中，真核生物中涉及的事件顺序和特定成分已被确定，并且基因表达中翻译控制的重要性已得到普遍认可。 然而，尽管这两个领域都取得了进展，但仍然存在巨大差距。 例如，几种真核起始因子的确切作用及其各个亚基的功能尚不清楚。 有鉴于此，很明显，模型系统（FV3 就是其中之一）对于增进我们对翻译和翻译控制的理解将是最重要的。 Chinchar 博士完全有资格开展这项研究。