Equipment to Expand Undergraduate Instruction in Cellular and Molecular Biology.

扩大细胞和分子生物学本科教学的设备。

• 批准号: 8750217
• 负责人: William Harvey
• 金额: $ 3.45万
• 依托单位: Earlham College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-05-15 至 1989-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8750217&HistoricalAwards=false
• 关键词: Equipment; Expand; Undergraduate; Instruction; Cellular

The Biology Department is involved in a four-phase program to expand the instrumentation of its Cellular and Molecular Biology laboratories. Phase one was completed by the institution; this project is covering the second phase which involves procurement of an ultracentrifuge, electrophoresis equipment, photographic equipment, a uv/visible spectrophotometer, a CO2 incubator, and an ion-free reagent water system. This equipment enables undergraduates to perform expanded laboratory experiments and individual projects in cell fractionation; protein, DNA and RNA separations; transfers of proteins and nucleic acids for testing and analysis (Southern, Northern, and Western blots); quantitative determination of proteins and nucleic acids; autoradiography; DNA sequencing; restriction digests of nucleic acids; and cloning of restriction fragments. A new course in Molecular Genetics is being offered, while major laboratory segments of Advanced Cell Biology and Developmental Biology are being expanded. Subtantial laboratory exercises also are being added to coursework in Microbiology, Genetics, Biochemistry, and Immunology. Students are gaining vastly expanded experience with current techniques and equipment used in Protein and Nucleic Acid Biochemistry and Molecular Genetics.

生物学部门参与了一个四相计划，以扩大其细胞和分子生物学实验室的仪器。 第一阶段由机构完成；该项目涵盖了第二阶段，该阶段涉及对超速离心设备，电泳设备，摄影设备，紫外/可见分光光度计，二氧化碳孵化器和无离子试剂水系统的采购。 该设备使本科生能够在细胞分馏中进行扩展的实验室实验和单个项目。蛋白质，DNA和RNA分离；用于测试和分析的蛋白质和核酸的转移（南部，北部和蛋白质印迹）； 蛋白质和核酸的定量测定； 放射自显影； DNA测序；核酸的限制消化；和限制片段的克隆。 提供了一个新的分子遗传学课程，而高级细胞生物学和发育生物学的主要实验室则正在扩展。 锥形实验室运动也被添加到微生物学，遗传学，生物化学和免疫学的课程中。 在蛋白质和核酸生物化学和分子遗传学中使用的当前技术和设备，学生正在获得大量扩大的经验。