The Mechanism and Regulation of Ovulation in Fish

鱼类排卵的机制和调控

• 批准号: 8718178
• 负责人: Frederick Goetz
• 金额: $ 5万
• 依托单位: University of Notre Dame
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-15 至 1989-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8718178&HistoricalAwards=false
• 关键词: Mechanism; Regulation; Ovulation; Fish

Past research implicates eicosanoids, the phosphatidylinositol/protein kinase C system and proteolytic enzymes in fish ovulation. The proposal research will increase our understanding of the role of these three mediators in fish ovulation. Specifically, the production of lipoxygense metabolites by brook trout and goldfish ovaries will be investigated using radio labeled precursor incorporation, followed by metabolite separation on high performance liquid chromatography. The production of primary prostaglandins during steroid-induced ovulation in yellow perch will be investigated using radioimmunoassay. An incorporation system, involving the labeling of follicular phosphatidylinositol by radioactive myoinositol, will be investigated so that future research can use this system to study the cycling of follicular inositol phosphates following agonist stimulation. Total follicular proteolytic activity thoughout maturation and ovulation will be followed in brook trout and goldfish using substrate polyacrylamide gel electrophoresis. Collagenolytic activity will be followed through ovulation in brook trout follicles using a specific substrate assay to determine the role of this proteases in the release of the eggs from the follicles. The goal is a better understanding of the endocrine processes regulating ovulation in fish.

过去的研究暗示了鱼类排卵中的类花生酸，磷脂酰肌醇/蛋白激酶C系统和蛋白水解酶。提案研究将提高我们对这三个介体在鱼排卵中的作用的理解。 具体而言，将使用布鲁克鳟鱼和金鱼卵巢生产Lipoxygense代谢产物，然后使用无线电标记的前体掺入来研究，然后在高性能液相色谱上进行代谢物分离。 在类固醇引起的黄鲈排卵过程中，将使用roadismunoassay研究原发性前列腺素的产生。 将研究一个通过放射性肌发醇标记卵泡磷脂性肌醇的结合系统，以便未来的研究可以利用该系统研究激动剂刺激后的卵泡肌醇磷酸盐的循环。 在小鳟鱼和金鱼中，使用底物聚丙烯酰胺凝胶电泳将遵循总卵泡蛋白水解活性。 使用特定的底物测定法，将通过在溪流鳟鱼卵泡中排卵来遵循胶原式活性，以确定该蛋白酶在卵泡中释放卵中的作用。 目的是更好地理解调节鱼排卵的内分泌过程。