Continuous Affinity Purification Using Confined and Supported Liquid Membranes

使用受限和支撑液膜进行连续亲和纯化

• 批准号: 8717768
• 负责人: Robert Coughlin
• 金额: $ 3.95万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-11-01 至 1989-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8717768&HistoricalAwards=false
• 关键词: Continuous; Affinity; Purification; Using; Confined

This is a one-year test-of-concept program to test the feasibility of a novel idea for continuous affinity chromatography/membrane process for a bioseparations. The idea is to use affinity ligands supported in a porous membrane with the two sides of the membrane operated as a chromatograph. Two major types of experiments will be done. First, affinity ligands in reversed micelles contained in a membrane are contacted with the target biomolecule (to be separated from the solution) in order to form a complex in the reversed micelle. The system is then eluted under various conditions: flowrates, pH, temperatures, concentrations, etc. to test for the best separation conditions.

这是一项为期一年的概念测试计划，旨在测试用于生物分离的连续亲和色谱/膜工艺的新想法的可行性。 这个想法是使用多孔膜上支持的亲和配体，膜的两侧用作色谱仪。 将进行两种主要类型的实验。 首先，膜中所含反胶束中的亲和配体与目标生物分子（待从溶液中分离）接触，以在反胶束中形成复合物。 然后在各种条件下洗脱系统：流速、pH、温度、浓度等，以测试最佳分离条件。