Structure, Binding, and Dynamics of Arabinogalactan- Proteins

阿拉伯半乳聚糖蛋白质的结构、结合和动力学

• 批准号: 8716179
• 负责人: Eugene Nothnagel
• 金额: $ 17.88万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-05-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8716179&HistoricalAwards=false
• 关键词: Structure; Binding; Dynamics; Arabinogalactan; Proteins

A class of macromolecules known as arabinogalactan-proteins (AGPs), that are present both at the outside surface of the plasma membrane and in the cell wall space of plant cells, will be investigated. Because AGPs have the ability to bind carbohydrates and are localized on the surface of the plasma membrane, they may play an important role at the membrane- cell wall interface in terms of cellular responses. The main objectives of the investigation are to compare the structure of AGPs on the cell wall with that of AGPs in plasma membrane, to study AGP interaction with some antigens, to characterize an enzyme that destroys the carbohydrate binding properties of plasma membrane AGPs, and to characterize the interaction between the AGPs and the plasma membrane with those in the cell wall. The project will increase our knowledge about the structural components of plasma membrane in plant cells and interaction of those components with the cell wall. Knowledge gained from this could eventually lead to a better understanding of cell adhesion, water retention, disease resistance and cell-cell recognition in plant cells.

将研究一类称为阿拉伯氏菌蛋白质（AGP）的大分子，它们都存在于质膜的外表面和植物细胞的细胞壁空间中。 由于AGP具有结合碳水化合物并位于质膜表面的能力，因此在细胞反应方面，它们可能在膜细胞壁界面上起重要作用。 研究的主要目的是将细胞壁上AGP的结构与质膜中AGP的结构进行比较，以研究与某些抗原的AGP相互作用，以表征破坏质膜AGP的碳水化合物结合特性的酶表征AGP和质膜与细胞壁中的相互作用。 该项目将增加我们对植物细胞中质膜结构成分以及这些成分与细胞壁的相互作用的了解。 从中获得的知识最终可能导致对植物细胞中细胞粘附，保留水，抗病性和细胞细胞识别的更好理解。