Synaptonemal Complexes and Meiotic Chromosome Metabolism in Yeast

酵母中的联会复合体和减数分裂染色体代谢

基本信息

批准号：
8711551
负责人：
Nancy Kleckner
金额：
$ 38.53万
依托单位：
Harvard University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1987
资助国家：
美国
起止时间：
1987-08-15 至 1991-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=8711551&HistoricalAwards=false
关键词：
Synaptonemal Complexes Meiotic Chromosome Metabolism

项目摘要

The proposed experimentation addresses the structure and function of the synaptonemal complexes that form during meiosis in yeast. The proteins present in the complex will be isolated and characterized. Antibody directed against the proteins will be raised and then used to investigate the structure of the complexes using standard immunocytological techniques. The genes encoding the proteins will also be isolated. In related experiments, Dr. Kleckner will isolate and characterize both the rad50 gene and its product, and then determine the intracellular location of the product. Regulation of the rad50 gene, especially during meiosis will also be examined. Meiotic cell division, or meiosis, is an event common to the life cycles of virtually all eukaryotic organisms. During meiosis, homologous chromosomes pair and recombine, then segregate to the daughter nuclei. As yet, remarkably little is understood about the biochemical mechanism of eukaryotic recombination or its regulation. Paired chromosomes with sites of contact, or synapses have been observed in the microscope. The occurrence of such synaptonemal complexes appears to correlate with recombination. Some yeast mutants, in particular those affected in the rad50 gene, display lowered frequencies of recombination. The proposed isolation and characterization of the rad50 gene, its product and the protein components of the synaptonemal complex will lay a solid foundation for understanding the molecular events of eukaryotic recombination.

所提出的实验解决了酵母减数分裂过程中形成的联会复合体的结构和功能。复合物中存在的蛋白质将被分离和表征。将产生针对蛋白质的抗体，然后使用标准免疫细胞学技术研究复合物的结构。编码蛋白质的基因也将被分离。在相关实验中，Kleckner博士将分离并表征rad50基因及其产物，然后确定产物在细胞内的位置。 rad50 基因的调节，特别是减数分裂期间的调节也将被检查。减数分裂细胞分裂或减数分裂是几乎所有真核生物生命周期中常见的事件。在减数分裂期间，同源染色体配对并重组，然后分离到子核。迄今为止，人们对真核重组的生化机制或其调节知之甚少。已在显微镜下观察到具有接触位点或突触的配对染色体。这种联会复合体的出现似乎与重组相关。一些酵母突变体，特别是那些受 rad50 基因影响的酵母突变体，表现出重组频率降低。所提出的rad50基因、其产物和联会复合体的蛋白质成分的分离和表征将为理解真核重组的分子事件奠定坚实的基础。