毛果杨GATA基因家族全基因组水平鉴定及表达分析

The GATA transcription factor gene family plays an important role in plant growth and development, cell differentiation and response to environmental changes. However, there is currently no report on the analysis of this gene family at the whole-genome level in woody plants. In this study, a systematic analysis was carried out on the information such as the number of GATA family members, gene structure, chromosomal location, phylogenetic evolution, physicochemical characteristics of encoded proteins and conserved motifs in Populus trichocarpa at the genome level. The results showed that the GATA family in Populus trichocarpa contains 39 genes, which are distributed on 15 chromosomes. Among them, 6 genes are located on chromosome 5, and the number of genes on chromosomes 9, 13 and 19 is 1, and there is no gene distribution on the remaining chromosomes. There are certain differences in the structure of each gene in this family and the basic characteristics of the encoded proteins, and they can be divided into 4 subfamilies. qRT - PCR studies showed that there are significant differences in the expression levels of each gene in the GATA family in the stems at different developmental stages, and salt stress has a significant impact on the expression characteristics of each gene. The above results indicate that after gene duplication in the GATA family of Populus trichocarpa, the gene structure and function have significantly differentiated, and some of the genes may play an important role in the secondary growth and salt stress response of Populus trichocarpa. This study lays a foundation for a comprehensive analysis of the biological functions of each member of the GATA family in Populus trichocarpa during its growth and development and in response to salt stress.

GATA转录因子基因家族在植物生长发育、细胞分化以及响应环境变化中具有重要作用。然而,目前在木本植物中尚无该基因家族全基因组水平的分析报导。本项研究从基因组水平对毛果杨GATA家族成员的数量、基因结构、染色体定位、系统进化、编码蛋白的理化特征和保守基序等信息进行了系统分析,结果表明,毛果杨GATA家族包含39个基因,共分布于15条染色体上,其中5号染色体上含有6个基因,9号、13号和19号染色体含有基因数量为1,其余染色上无基因分布。该家族各基因的结构与编码蛋白的基本特性均存在一定异性,可分成4个亚族。qRT-PCR研究表明,GATA家族各基因在不同发育阶段的茎部表达量存在明显差异,且盐胁迫对各基因的表达特性影响显著。以上结果表明,毛果杨GATA家族基因在复制后,基因的结构与功能产生了明显分化,其中部分基因在毛果杨次生生长与盐胁迫响应中可能具有重要作用。本项研究为全面解析毛果杨GATA家族各成员在其生长发育与盐胁迫响应中的生物学功能奠定了基础。