Specific Reactions of RSNO, HSNO, and HNO and Their Applications in the Design of Fluorescent Probes.

Nitric oxide (NO)-derived species play essential roles in regulating cellular responses. Among these species, S-nitrosothiols (including RSNO and HSNO) and nitroxyl (HNO) are especially interesting. Owing to their high reactivity and short survival time, the detection of these molecules in biological settings can be challenging. In this regard, much effort has been invested in exploring novel reactions of RSNO/HSNO/HNO and applying these reactions to develop fluorescence probes. Herein, reported specific reactions of RSNO/HSNO/HNO are summarized and strategies used in the design of fluorescent probes are illustrated. The properties and potential problems of representative probes are also discussed.

一氧化氮（NO）衍生的物质在调节细胞反应中起着至关重要的作用。在这些物质中，S - 亚硝基硫醇（包括RSNO和HSNO）和亚硝酰（HNO）尤其令人关注。由于它们的高反应活性和短存活时间，在生物环境中检测这些分子可能具有挑战性。在这方面，人们投入了大量努力来探索RSNO/HSNO/HNO的新型反应，并将这些反应应用于开发荧光探针。在此，对已报道的RSNO/HSNO/HNO的特定反应进行了总结，并阐述了荧光探针设计中所使用的策略。还讨论了代表性探针的性质和潜在问题。