Griflola frondosa (GF) produces significant antidepressant effects involving AMPA receptor activation in mice.

Context:Griflola frondosa (Fr) S.F. Gray (Meripilaceae) (GF) is a medical mushroom, and its regulation of the immune system is of interest for the treatment of mood disorders. α-Amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors are the central mediator for the treatment of depression. Objective: This study examines the antidepressant effects of GF and the role of AMPA in these antidepressant effects. Materials and methods: The CD-1 mice were fed with GF- or Pleurotus ostreatus [(Jacq.: Fr) Kumm (Pleurotaceae)] (PO)-containing food for 1 day or 5 days. The antidepressant effects was determined in the tail suspension test (TST), forced swim test (FST), and open field test (OFT). The involvement of AMPA receptors was determined by the application of the AMPA-specific blocker GYKI 52466. Results: Treatments with 20%, 33% or 50% of GF-containing food significantly decreased the immobility time (63.6, 56.9, and 52.0% in TST; and 50.8, 43.2, and 38.2% in FST) after 1 day and (62.3, 51.8, and 52.8% in TST; and 49.5, 45.1, and 40.3% in FST) after 5 days. GF-containing food did not cause hyperactive effects in the OFT. The antidepressant effects of the 33% of GF-containing food (down-to 51.3% in 1-day TST and 46.8% in 5-day FST) were significantly stronger than that of the 33% of PO-containing food (down-to 85.5% in 1-day TST and 82.0% in 5-day FST). AMPA-specific blocker GYKI 52466 was able to block the antidepressant effects of the GF-containing food. Conclusion: GF demonstrated the potential as a safe medical food supplement for the patient with depression.

背景：灰树花（Griflola frondosa (Fr) S.F. Gray，多孔菌科）是一种药用蘑菇，其对免疫系统的调节作用在治疗情绪障碍方面具有研究价值。α - 氨基 - 3 - 羟基 - 5 - 甲基 - 4 - 异恶唑 - 丙酸（AMPA）受体是治疗抑郁症的核心介质。 目的：本研究探讨灰树花的抗抑郁作用以及AMPA在这些抗抑郁作用中的角色。 材料和方法：给CD - 1小鼠喂食含灰树花或平菇[（Jacq.: Fr）Kumm（侧耳科）]的食物，持续1天或5天。通过悬尾实验（TST）、强迫游泳实验（FST）和旷场实验（OFT）来测定抗抑郁效果。通过应用AMPA特异性阻断剂GYKI 52466来确定AMPA受体的参与情况。 结果：喂食含20%、33%或50%灰树花的食物，在1天后显著减少了不动时间（在TST中分别为63.6%、56.9%和52.0%；在FST中分别为50.8%、43.2%和38.2%），在5天后（在TST中分别为62.3%、51.8%和52.8%；在FST中分别为49.5%、45.1%和40.3%）也是如此。含灰树花的食物在旷场实验中没有引起过度活跃的效果。含33%灰树花的食物的抗抑郁效果（在1天的TST中降至51.3%，在5天的FST中降至46.8%）明显强于含33%平菇的食物（在1天的TST中降至85.5%，在5天的FST中降至82.0%）。AMPA特异性阻断剂GYKI 52466能够阻断含灰树花食物的抗抑郁效果。 结论：灰树花显示出作为抑郁症患者安全的药用食品补充剂的潜力。