Hyaluronic Acid-Modified Multifunctional Q-Graphene for Targeted Killing of Drug-Resistant Lung Cancer Cells

Considering the urgent need to explore multifunctional drug delivery system for overcoming multidrug resistance, we prepared a new nanocarbon material Q-Graphene as a nanocarrier for killing drug-resistant lung cancer cells. Attributing to the introduction of hyaluronic acid and rhodamine B isothiocyanate (RBITC), the Q-Graphene-based drug delivery system was endowed with dual function of targeted drug delivery and fluorescence imaging. Additionally, doxorubicin (DOX) as a model drug was loaded on the surface of Q-Graphene via pi-pi stacking. Interestingly, the fluorescence of DOX was quenched by Q-Graphene due to its strong electron-accepting capability, and a significant recovery of fluorescence was observed, while DOX was released from Q-Graphene. Because of the RBITC labeling and the effect of fluorescence quenching/restoring of Q-Graphene, the uptake of nanoparticles and intracellular DOX release can be tracked. Overall, a highly promising multifunctional nanoplatform was developed for tracking and monitoring targeted drug delivery for efficiently killing drug-resistant cancer cells.

考虑到迫切需要探索多功能药物递送系统以克服多药耐药性，我们制备了一种新型纳米碳材料Q - 石墨烯作为纳米载体来杀死耐药肺癌细胞。由于引入了透明质酸和异硫氰酸罗丹明B（RBITC），基于Q - 石墨烯的药物递送系统被赋予了靶向给药和荧光成像的双重功能。此外，阿霉素（DOX）作为一种模型药物通过π - π堆积负载在Q - 石墨烯表面。有趣的是，由于Q - 石墨烯具有很强的电子接受能力，DOX的荧光被Q - 石墨烯猝灭，并且当DOX从Q - 石墨烯释放时，观察到荧光显著恢复。由于RBITC标记以及Q - 石墨烯的荧光猝灭/恢复作用，可以追踪纳米颗粒的摄取和细胞内DOX的释放。总体而言，开发了一种非常有前景的多功能纳米平台，用于追踪和监测靶向给药，以高效杀死耐药癌细胞。