Red emission ratio fluorescent probe for the activity of vanin-1 and imaging in vivo

Pantetheinase, also known as Vanin-1, catalyzes pantetheine to decompose into the precursor of CoA - pantothenic acid and aminothiol cysteamine. Studies have shown that Vanin-1 plays an important role in many important physiological pathologies. In this paper, a new red emission ratio fluorescent probe DCM-PA (I-640 nm/I-564 run) has been implemented to detect the activity of Vanin-1 in cells and vivo. DCM-PA has short response time (30 min), high selectivity and low sensitivity (DL =0.69 ng/mL). Also, we have applied DCM-PA for imaging in cells and mice, and the results have indicated that the probe has a non-negligible potential for monitoring the activity of Vanin-1 in situ, benefiting further to study the role of Vanin-1 in physiology and pathology. In addition, the up-regulation of this enzyme by starvation confirmed the inevitable connection between diabetes and abnormal expression of Vanin-1.

泛酰巯基乙胺酶，又称血管非炎性蛋白-1（Vanin-1），可催化泛酰巯基乙胺分解为辅酶A的前体——泛酸和氨基硫醇半胱胺。研究表明，Vanin-1在许多重要的生理病理过程中发挥着重要作用。本文构建了一种新型红色发射比率荧光探针DCM - PA（I - 640 nm/I - 564 nm），用于检测细胞及活体中Vanin - 1的活性。DCM - PA响应时间短（30分钟）、选择性高且灵敏度低（检测限DL = 0.69 ng/mL）。此外，我们将DCM - PA应用于细胞和小鼠成像，结果表明该探针在原位监测Vanin - 1活性方面具有不可忽视的潜力，有助于进一步研究Vanin - 1在生理和病理中的作用。另外，饥饿状态下该酶的上调证实了糖尿病与Vanin - 1异常表达之间存在必然联系。