Xenopus laevis Einstecks.

INTRODUCTION"Einstecks" refers to a procedure for placing a piece of tissue into the blastocoel of an early gastrula, in order to assess the inductive potential of the introduced tissue. The foreign tissue adheres to surrounding tissue and becomes incorporated into the host embryo. This simple transplant procedure, which is described here, has been used to assess which types of axial tissue can be induced by different regions of the mesendoderm or ectoderm, and how forced expression of genes or treatment with various factors can alter this potential.

引言 “Einstecks”是指将一块组织放入原肠胚早期的囊胚腔的一种操作程序，目的是评估所引入组织的诱导潜能。外来组织会黏附在周围组织上，并融入宿主胚胎。这里所描述的这种简单的移植操作程序已被用于评估中内胚层或外胚层的不同区域能够诱导哪些类型的轴性组织，以及基因的强制表达或用各种因子处理如何改变这种潜能。