GC/MS-based metabolomics approach to identify biomarkers differentiating survivals from death in crucian carps infected by Edwardsiella tarda

Microbial disease problems constitute the largest single cause of economic losses in aquaculture. An understanding of immune system in aquaculture animals how to function in defense against bacterial infections is especially important to control these diseases and improve food quality and safety. In the present study, we use a crucian carp model to explore which pathways and metabolites are crucial for the defense against infection caused by Edwardsiella tarda EIB202. We establish the metabolic profile of crucian carps and then compare the metabolic difference between survivals and dead fish by self-control. We identify elevating unsaturated fatty acid biosynthesis and decreasing fructose and mannose metabolism as the most key pathways and increasing palmitic acid and decreasing D-mannose as the most crucial metabolites differentiating survivals from death in these fish infected by E. tarda. Our findings highlight the importance of metabolic strategy against bacterial infections. (C) 2014 Elsevier Ltd. All rights reserved.

微生物疾病问题是水产养殖中经济损失的最大单一原因。了解水产养殖动物的免疫系统如何防御细菌感染，对于控制这些疾病以及提高食品质量和安全尤为重要。在本研究中，我们利用鲫鱼模型来探究哪些途径和代谢物对防御迟缓爱德华氏菌EIB202引起的感染至关重要。我们建立了鲫鱼的代谢图谱，然后通过自身对照比较了存活鱼和死亡鱼之间的代谢差异。我们确定不饱和脂肪酸生物合成增加以及果糖和甘露糖代谢降低是最关键的途径，棕榈酸增加和D - 甘露糖减少是区分感染迟缓爱德华氏菌的这些鱼中存活鱼和死亡鱼的最关键代谢物。我们的研究结果强调了针对细菌感染的代谢策略的重要性。(C) 2014爱思唯尔有限公司。保留所有权利。