REGULATION OF VASOACTIVE-INTESTINAL-PEPTIDE EXPRESSION IN SYMPATHETIC NEURONS IN CULTURE AND AFTER AXOTOMY - THE ROLE OF CHOLINERGIC DIFFERENTIATION FACTOR LEUKEMIA INHIBITORY FACTOR

Vasoactive intestinal peptide (VIP) expression increases in sympathetic neurons when they are grown in dissociated cell or explant cultures and when they are axotomized in vivo. In dissociated cell culture, the magnitude of the VIP increase was reduced when nonneuronal cells were removed and medium conditioned by ganglionic nonneuronal cells increased VIP in neuron-enriched cultures. Antiserum against cholinergic differentiation factor (also leukemia inhibitory factor; CDF/LIF), but not against ciliary neurotrophic factor, immunoprecipitated this activity. Medium conditioned by sympathetic ganglion explants also contained a VIP-stimulatory molecule that was immunoprecipitated by CDF/LIF antiserum, and CDF/LIF antiserum partially blocked VIP induction in explants. CDF/LIF mRNA was increased in dissociated cell cultures, in ganglion explants and in vivo after axotomy. Our results suggest that CDF/LIF released from ganglionic nonneuronal cells plays an important role in regulating VIP after axotomy. (C) 1994 John Wiley and Sons, Inc.

当交感神经元在离散细胞培养或外植体培养中生长，以及在体内轴突切断时，血管活性肠肽（VIP）的表达会增加。在离散细胞培养中，当去除非神经元细胞时，VIP增加的幅度会降低，并且神经节非神经元细胞所调节的培养基会增加富含神经元的培养物中的VIP。抗胆碱能分化因子（也是白血病抑制因子；CDF/LIF）的抗血清，而非抗睫状神经营养因子的抗血清，能使这种活性免疫沉淀。交感神经节外植体所调节的培养基也含有一种VIP刺激分子，该分子可被CDF/LIF抗血清免疫沉淀，并且CDF/LIF抗血清可部分阻断外植体中VIP的诱导。在离散细胞培养、神经节外植体以及体内轴突切断后，CDF/LIF mRNA会增加。我们的研究结果表明，神经节非神经元细胞释放的CDF/LIF在轴突切断后对VIP的调节起着重要作用。（C）1994年约翰威立父子公司