TRANSCRIPTOME PROFILING OF COLD RESISTANT GENES IN LIRIODENDRON CHINENSE (HEMSL.) SARG

Transcriptome sequencing technology was used to analyze differential transcript expressions of.Liriodendron chinense of two provenances in different temperature, and a lot of unigenes were obtained..Digital gene expression profiling technology was used to select differentially expressed genes. A number of.differentially expressed genes were annotated on GO classification and pathway enrichment analysis.metabolic pathways. Some metabolic pathways are associated with cold resistance including plant hormone.signaling, unsaturated fatty acid biosynthesis, carbohydrate metabolism etc. A group of differentially.expressed unigenes related to plant cold resistance were found including calcium signaling pathway,.cold-response transcription factors, and genes maintaining membrane stability and adjusting osmotic balance..Low temperature stress experiment was carried on L. chinense and digital gene expression profiling.technology was used to select differentially expressed cold resistant genes. Twenty six unigenes were obtained.including cold-response transcription factors HSP, NAC etc., and genes maintaining membrane stability and.adjusting osmotic balance, for example, β-amylase. Real time PCR technology was used to verify the.expression characters of differentially expressed genes. This research provides basis for study cold resistance.mechanism of Liriodendron at gene level, and also provides theoretical basis for molecular breeding.

利用转录组测序技术分析了两个种源的鹅掌楸在不同温度下的差异转录表达，获得了大量的单基因。利用数字基因表达谱技术筛选差异表达基因。一些差异表达基因在基因本体（GO）分类和通路富集分析代谢途径上进行了注释。一些代谢途径与抗寒性相关，包括植物激素信号转导、不饱和脂肪酸生物合成、碳水化合物代谢等。发现了一组与植物抗寒相关的差异表达单基因，包括钙信号通路、冷响应转录因子以及维持膜稳定性和调节渗透平衡的基因。对鹅掌楸进行低温胁迫实验，并利用数字基因表达谱技术筛选差异表达的抗寒基因，获得了26个单基因，包括冷响应转录因子热休克蛋白（HSP）、NAC等，以及维持膜稳定性和调节渗透平衡的基因，例如β - 淀粉酶。利用实时荧光定量PCR技术验证差异表达基因的表达特性。本研究为从基因水平研究鹅掌楸的抗寒机制提供了依据，也为分子育种提供了理论基础。