蕙兰CfCIN基因克隆及其功能分析

Using Cymbidium f aberi Rolfe as the experimental material, the CIN homologous gene of the TCP family was cloned by RT-PCR technology. The open reading frame is 1161 bp long and encodes 386 amino acids, and it was named CfCIN (GenBank accession number KJ956809). In order to further analyze the function of CfCIN, a plant expression vector was constructed, and the leaves of Saintpaulia ionantha were transformed by the Agrobacterium-mediated method. Transformed plants were obtained and the traits of the transgenic plants were analyzed. The results showed that compared with the leaves of wild-type Saintpaulia ionantha, the leaves of the transgenic plants were larger, changing from round to oval, the leaf margins changed from flat and smooth to notched and slightly curled backward, the leaf veins were obvious, the petioles were red, and the changes in the shape of floral organs were not obvious. The research indicates that CfCIN may be involved in regulating the morphogenesis of plant leaves.

以蕙兰（Cymbidium f aberi Rolfe）为试验材料，采用RT‐PCR技术克隆了TCP家族的CIN同源基因，开放阅读框长1161 bp ，编码386个氨基酸，将其命名为CfCIN（GenBank登录号为KJ956809）。为进一步分析CfCIN的功能，构建了植物表达载体，采用农杆菌介导法转化非洲紫罗兰叶片，获得了转化植株并对转基因植株进行了性状分析。结果显示：与野生型非洲紫罗兰叶片相比，转基因植株的叶片更大，由圆形变为卵圆形，叶缘由平整光滑变为有缺刻且稍向后卷曲，叶脉明显，叶柄红，花器官形状变化不明显。研究表明，CfCIN可能参与调控植物叶片的形态建成。