Curvature-dependent recognition of ethanolamine phospholipids by duramycin and cinnamycin

Duramycin is a 19-amino-acid tetracyclic lantibiotic closely related to cinnamycin (Ro09-0198), which is known to bind phosphatidylethanolamine ( PE). The lipid specificity of duramycin was not established. The present study indicates that both duramycin and cinnamycin exclusively bind to ethanolamine phospholipids ( PE and ethanolamine plasmalogen). Model membrane study indicates that the binding of duramycin and cinnamycin to PE-containing liposomes is dependent on membrane curvature, i.e., the lantibiotics bind small vesicles more efficiently than large liposomes. The binding of the lantibiotics to multilamellar liposomes induces tubulation of membranes, as revealed by electron microscopy and small-angle x-ray scattering. These results suggest that both duramycin and cinnamycin promote their binding to the PE-containing membrane by deforming membrane curvature.

杜拉霉素是一种19个氨基酸的四环羊毛硫抗生素，与肉桂霉素（Ro09 - 0198）密切相关，已知肉桂霉素可结合磷脂酰乙醇胺（PE）。杜拉霉素的脂质特异性尚未确定。本研究表明，杜拉霉素和肉桂霉素都只与乙醇胺磷脂（PE和乙醇胺缩醛磷脂）结合。模型膜研究表明，杜拉霉素和肉桂霉素与含PE的脂质体的结合取决于膜曲率，即羊毛硫抗生素与小囊泡的结合比大脂质体更有效。电子显微镜和小角X射线散射显示，羊毛硫抗生素与多层脂质体的结合会诱导膜形成管状结构。这些结果表明，杜拉霉素和肉桂霉素都通过改变膜曲率来促进它们与含PE的膜的结合。