Actinopolymorpha rutila sp nov., isolated from a forest soil

A Gram-positive, aerobic actinomycete, designated strain YIM 45725(T), was isolated from a forest soil sample in Yunnan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was a member of the family Nocardioidaceae and was related most closely to Actinopolymorpha singaporensis IM 7744(T) (99.4% 16S rRNA gene sequence similarity). Chemotaxonomic data, including cell-wall components, menaquinones and polar lipids, supported the affiliation of strain YIM 45725(T) to the genus Actinopolymorpha. The results of physiological and biochemical investigations and DNA-DNA hybridization experiments demonstrated that strain YIM 45725(T) differed from the single recognized species of the genus Actinopolymorpha. Strain YIM 45725(T) is therefore considered to represent a novel species of the genus Actinopolymorpha, for which the name Actinopolymorpha rutila sp. nov. is proposed. The type strain is YIM 45725(T) (=CCTCC AA 206004(T) =DSM 18448(T)).

一株革兰氏阳性、需氧放线菌，命名为菌株YIM 45725(T)，从中国云南省的一份森林土壤样本中分离得到。基于16S rRNA基因序列的系统发育分析表明，该分离株是诺卡氏菌科（Nocardioidaceae）的成员，与新加坡放线多态菌（Actinopolymorpha singaporensis）IM 7744(T)亲缘关系最为密切（16S rRNA基因序列相似性为99.4%）。化学分类学数据，包括细胞壁成分、甲基萘醌和极性脂类，支持菌株YIM 45725(T)归属于放线多态菌属（Actinopolymorpha）。生理生化研究结果以及DNA - DNA杂交实验表明，菌株YIM 45725(T)不同于该属已被确认的单一物种。因此，菌株YIM 45725(T)被认为代表放线多态菌属（Actinopolymorpha）的一个新种，其名称被提议为朱红放线多态菌（Actinopolymorpha rutila sp. nov.）。模式菌株为YIM 45725(T)（=CCTCC AA 206004(T) = DSM 18448(T)）