Sp1 elements in <em>SULT2B1b</em> promoter and 5′-untranslated region of mRNA: Sp1/Sp2 induction and augmentation by histone deacetylase inhibition

The steroid/sterol sulfotransferase gene () encodes for two isozymes of which one (SULT2B1b) sulfonates cholesterol and is selectively expressed in skin. The human gene contains neither a TATAAA nor a CCAAT motif upstream of the coding region for SULT2B1b; however, this area is GC-rich. Of five Sp1 elements identified two had regulatory activity utilizing immortalized human keratinocytes: one element is located above the ostensible transcription initiation site, whereas the other is located within the 5′-untranslated region of the SULT2B1b mRNA. Sp1 and Sp2 transcription factors identified by supershift analyses induced reporter gene activity, an effect markedly augmented by histone deacetylase inhibition.

类固醇/甾醇磺基转移酶基因（）编码两种同工酶，其中一种（SULT2B1b）使胆固醇磺化，并在皮肤中选择性表达。人类基因在SULT2B1b编码区上游既无TATAAA也无CCAAT基序；然而，该区域富含GC。在鉴定出的5个Sp1元件中，有2个在利用永生化人角质形成细胞时具有调节活性：一个元件位于表面上的转录起始位点上方，而另一个位于SULT2B1b mRNA的5′ - 非翻译区。通过超迁移分析鉴定的Sp1和Sp2转录因子诱导报告基因活性，这种作用因组蛋白去乙酰化酶抑制而显著增强。