Primers and probe for detecting EGFR gene mutation,probe, kit thereof, and application method of kit

The invention relates to the field of gene mutation detection, and specifically relates to primers and a probe used for detecting EGFR gene mutation, a kit thereof, and an application method of the kit. With the kit provided by the invention, a sample containing 0.1-1% of EGFR gene mutation DNA can be detected; and accurate genotyping can be carried out upon a sample containing 10% of EGFR No. 19 exon gene mutation DNA through sequencing. The sensitivity of the kit provided by the invention is high, wherein a minimum detection limit is 5-copy gene. With the kit, qualitative detection can be carried out upon EGFR gene mutation. Different sensitivity reference ranges can be provided for assisting clinical determinations of mutation amount volumes, such that final treatment programs can be provided according to different mutations.

本发明涉及基因突变检测领域，具体涉及用于检测EGFR基因突变的引物和探针、其试剂盒以及该试剂盒的应用方法。利用本发明提供的试剂盒，可检测出EGFR基因突变DNA含量为0.1% - 1%的样本；对于EGFR第19外显子基因突变DNA含量为10%的样本，可通过测序进行准确的基因分型。本发明提供的试剂盒灵敏度高，最低检测限为5拷贝基因。利用该试剂盒可对EGFR基因突变进行定性检测，还能提供不同的灵敏度参考范围，以辅助临床判断突变数量，从而根据不同的突变情况制定最终的治疗方案。