Development of rapid and simple experimental and in silico serotyping systems for Citrobacter.

Members of the genus Citrobacter are important opportunistic pathogens responsible for high mortality rate. Therefore, in this study, we aimed to develop efficient and accurate Citrobacter typing schemes for clinical detection and epidemiological surveillance. Using genomic and experimental analyses, we located the O-antigen biosynthesis gene clusters in Citrobacter genome for the first time, and used comparative genomic analyses to reveal the specific genes in different Citrobacter serotypes. Based on the specific genes in O-antigen biosynthesis gene clusters of Citrobacter, we established experimental and in silico serotyping systems for this bacterium. Both serotyping tools are reliable, and our observations are biologically and clinically relevant for understanding and managing Citrobacter infection.

柠檬酸属的成员是负责高死亡率的重要机会病原体。因此，在这项研究中，我们旨在开发有效，准确的柠檬酸分类方案，以进行临床检测和流行病学监测。 使用基因组和实验分析，我们首次将O-抗原生物合成基因簇找到了基因组，并使用了比较基因组分析来揭示不同柠檬酸杆菌血清型中的特定基因。 基于柠檬酸杆菌O-抗原生物合成基因簇中的特定基因，我们在该细菌的实验和硅血清分型系统中建立了。 两种血清分型工具都是可靠的，我们的观察结果在生物学和临床上与理解和管理柠檬酸杆菌感染相关。