Elevated expression of HMGB1 in squamous-cell carcinoma of the head and neck and its clinical significance

Purpose HMGB1 overexpression has been reported in a variety of human cancers However the role of HMGB1 in squamous cell carcinoma of the head and neck (SCCHN) remains unclear The aim of the present investigation was to analyse HMGB1 protein expression in both SCCHN tissue and cell levels and to assess its prognostic significance in SCCHNMethods HMGB1 protein expression in 103 primary SCCHN tissue specimens was analysed by immunohistochemistry and correlated with clinicopathological parameters and patient outcome Additionally HMGB1 protein expression was evaluated in cell level by Western blottingResults By Western blotting analysis all the 5 SCCHN cell lines overexpressed HMGB1 protein whereas the non transformed immortalised cell line NP 69 had relatively weak HMGB1 protein expression immunohistochemical staining revealed that HMGB1 protein was detected in 91 (91/103 88 3%) primary tumour samples but only in 7 (7/16 43 75%) adjacent non carcinoma samples (p

目的：据报道，高迁移率族蛋白B1（HMGB1）在多种人类癌症中过度表达。然而，HMGB1在头颈部鳞状细胞癌（SCCHN）中的作用仍不清楚。本研究的目的是分析HMGB1蛋白在SCCHN组织和细胞水平的表达，并评估其在SCCHN中的预后意义。 方法：采用免疫组织化学方法分析103例原发性SCCHN组织标本中HMGB1蛋白的表达，并与临床病理参数及患者预后相关联。此外，通过蛋白质印迹法在细胞水平评估HMGB1蛋白的表达。 结果：通过蛋白质印迹分析，所有5种SCCHN细胞系均过度表达HMGB1蛋白，而未转化的永生化细胞系NP69的HMGB1蛋白表达相对较弱。免疫组织化学染色显示，在91例（91/103，88.3%）原发性肿瘤样本中检测到HMGB1蛋白，但在仅7例（7/16，43.75%）相邻的非癌组织样本中检测到（p<...此处原文似乎不完整）