Nasal instillation of probiotic extracts inhibits experimental allergic rhinitis

Aim: Allergic rhinitis (AR) is a common disease. The therapeutic efficacy of AR needs to be improved. This study aims to evaluate the effects of local administration of probiotic extracts on inhibiting experimental AR. Methods: Epithelial cells (ECs) were primed by exposing to Clostridium butyricum extracts (CBe) in the culture to upregulate the expression of IL-10. A mouse AR model was developed to assess the therapeutic potential of CBe in AR. Results: CBe markedly induced the expression of IL-10 in ECs. Co-culture of naive B cells with CBe-primed ECs significantly increased IL-10 expression in the B cells (iB10 cells). The iB10 cells showed immune suppressive function in suppressing effector CD4(+) T-cell proliferation. Treatment with nasal drops containing CBe efficiently inhibited experimental AR in mice. Conclusion: Local administration of CBe can efficiently inhibit experimental AR.

目的：过敏性鼻炎（AR）是一种常见疾病。AR的治疗效果需要提高。本研究旨在评估局部应用益生菌提取物对抑制实验性AR的作用。方法：通过在培养过程中使上皮细胞（ECs）暴露于丁酸梭菌提取物（CBe）来诱导其上调白细胞介素 - 10（IL - 10）的表达。建立小鼠AR模型以评估CBe在AR中的治疗潜力。结果：CBe显著诱导ECs中IL - 10的表达。将未致敏的B细胞与CBe诱导的ECs共培养，可显著增加B细胞（iB10细胞）中IL - 10的表达。iB10细胞在抑制效应CD4(+) T细胞增殖方面显示出免疫抑制功能。用含CBe的滴鼻剂治疗可有效抑制小鼠的实验性AR。结论：局部应用CBe可有效抑制实验性AR。