OsBELL4A调控水稻WOX家族基因表达及水稻幼苗生长和耐逆

The root system not only plays an important supporting role for the above-ground part, but is also the main organ for plants to absorb water and nutrients. In order to study the functions of BELL family factors in plant growth and development and in response to stress, four BELL4 homologous genes in rice were identified by comparison with the Arabidopsis sequence. Their promoters contain elements such as ABRE, GARE, ERE, ARE and DRE that respond to stress and hormone signals. Real-time quantitative PCR analysis in rice roots showed that these four BELL4 homologous genes are not only induced by stress such as drought, low temperature and salt, but are also regulated by plant hormones such as ethylene, gibberellin and abscisic acid, indicating that these genes may respond to multiple hormones and stress. Further analysis of the phenotypes of OsBELIAA interference materials found that suppressing the OsBELLAA gene can promote the expression level of the WOX family genes and inhibit the growth of the primary root at the rice seedling stage, but increase the number of crown roots. These results indicate that the rice BELL4 homologous gene OsBELL4A may have an important regulatory function in rice root development and in response to stress.

根系不仅对地上部分起着重要的支撑作用,也是植物吸收水分和养分的主要器官。为了研究BELL家族因子在植物生长发育及逆境胁迫应答中的功能,通过与拟南芥序列对比,鉴定到水稻4个BELL4同源基因,它们的启动子含有ABRE、GARE、ERE、ARE和DRE等应答逆境胁迫和激素信号的元件。在水稻根中进行实时定量PCR分析表明,这4个BELL4同源基因不仅受到干旱、低温以及盐等逆境胁迫诱导,而且还受到植物激素乙烯、赤霉素以及脱落酸的调控,表明这些基因可能应答多种激素及逆境胁迫。进一步分析OsBELIAA干扰材料表型发现,抑制OsBELLAA基因能够促进WOX家族基因的表达水平,并抑制水稻苗期初生根生长,但使冠根数量增多。这些结果表明水稻BELL4同源基因OsBELL4A在水稻根系发育及逆境胁迫应答中可能具有重要的调控功能。