CD24 tracks divergent pluripotent states in mouse and human cells.

CD24 tracks divergent pluripotent states in mouse and human cells.

Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture. Characterizing the cellular stages that lead to induced reprogramming is of much interest and cell surface markers could offer unique advantages for this. Here the authors use surface proteomics and discover CD24 as a marker that tracks reprogramming-responsive cells and enables the analysis and enrichment of transgene-dependent and -independent induced pluriopotent stem cells.

重编程是一个动态过程，可能导致多种多能细胞类型从不同途径产生。细胞表面蛋白表达是对重编程和多能性进行分类的一种特别理想的工具，因为它能够对活细胞进行可靠的定量和富集。在此，我们利用细胞表面蛋白质组学来探究小鼠细胞重编程动态，并发现CD24可作为一种标志物，追踪重编程响应细胞的出现，同时能够在后期对转基因依赖型（F类）和非依赖型（传统型）诱导多能干细胞（iPSCs）进行分析和富集。此外，CD24可用于在小鼠多能培养中区分上胚层干细胞（EpiSCs）和胚胎干细胞（ESCs）。重要的是，受调控的CD24表达在人类多能干细胞（PSCs）中是保守的，可追踪人类ESCs向更原始样PSC状态的转变。因此，CD24是一种在重编程和标准多能培养中追踪不同状态的保守标志物。 对导致诱导重编程的细胞阶段进行表征是非常令人感兴趣的，而细胞表面标志物可能为此提供独特优势。在此，作者利用表面蛋白质组学并发现CD24可作为一种标志物，追踪重编程响应细胞，并能够对转基因依赖型和非依赖型诱导多能干细胞进行分析和富集。