Naive and Primed Pluripotent States

Naive and Primed Pluripotent States

After maternal predetermination gives way to zygotic regulation, a ground state is established within the mammalian embryo. This tabula rasa for embryogenesis is present only transiently in the preimplantation epiblast. Here, we consider how unrestricted cells are first generated and then prepared for lineage commitment. We propose that two phases of pluripotency can be defined: naive and primed. This distinction extends to pluripotent stem cells derived from embryos or by molecular reprogramming ex vivo.

在母体预决定让位于合子调控之后，哺乳动物胚胎内建立起一种基础状态。这种胚胎发生的白板状态仅在着床前的上胚层中短暂存在。在此，我们思考无限制的细胞是如何首先产生，然后又如何为谱系定型做好准备的。我们提出，多能性可被定义为两个阶段：原始态和激发态。这种区分延伸到源自胚胎的多能干细胞或通过体外分子重编程获得的多能干细胞。