Effects of glucose and insulin on the H9c2 (2-1) cell proliferation may be mediated through regulating glucose transporter 4 expression

Background The change of glucose transporter 4 (GLUT4) expression could influence glucose uptake in the myocardial cells and then effect myocardial metabolism, which maybe one of the factor for the diabetes cardiovascular disease. This study aimed to explore the influence of glucose and insulin at different concentrations on H9c2 (2-1) cell proliferation and its GLUT4 expression in vitro, and evaluate the correlation between myocardial cells proliferation and GLUT4 expression. This might be helpful for understanding the relationship between glucose metabolism and cardiovascular disease.Methods According to glucose concentrations in culture medium, cultured H9c2 rat myocardial cells were divided into five groups: control group (NC, glucose concentration 5.0 mnnol/L), low glucose group (LG, glucose concentration 0.1 mmol/L), high glucose group 1 (HG1, glucose concentration 10 mmol/L), high glucose group 2 (HG2, glucose concentration 15 mmol/L), high glucose group 3 (HG3, glucose concentration 20 mmol/L). Then according to different insulin concentrations in culture medium, each group was further divided into two subgroups: normal insulin subgroup (INSc, insulin concentration 3.8 nnU/L), high insulin subgroup (INSh, insulin concentration 7.6 mU/L). H9c2 (2-1) cells were cultured for 1, 2, 3 days, the proliferation of cells were assayed by cell counting Kit-8 assay, the expressions of GLUT4 mRNA and protein were detected with RT-PCR and Western Blotting technique, and the relation between myocardial cells proliferation and GLUT4 expression was evaluated.Results Compared with NC group, cell proliferation (OD value) was lower in LG, HG2, HG3 group but higher in HG1 group on the second and the third day (P

背景 葡萄糖转运蛋白4（GLUT4）表达的变化可影响心肌细胞对葡萄糖的摄取，进而影响心肌代谢，这可能是糖尿病心血管疾病的因素之一。本研究旨在探讨不同浓度的葡萄糖和胰岛素对体外培养的H9c2（2 - 1）细胞增殖及其GLUT4表达的影响，并评估心肌细胞增殖与GLUT4表达之间的相关性。这可能有助于理解葡萄糖代谢与心血管疾病之间的关系。 方法 根据培养基中的葡萄糖浓度，将培养的H9c2大鼠心肌细胞分为五组：对照组（NC，葡萄糖浓度5.0 mmol/L）、低糖组（LG，葡萄糖浓度0.1 mmol/L）、高糖组1（HG1，葡萄糖浓度10 mmol/L）、高糖组2（HG2，葡萄糖浓度15 mmol/L）、高糖组3（HG3，葡萄糖浓度20 mmol/L）。然后根据培养基中不同的胰岛素浓度，每组又进一步分为两个亚组：正常胰岛素亚组（INSc，胰岛素浓度3.8 nU/L）、高胰岛素亚组（INSh，胰岛素浓度7.6 mU/L）。H9c2（2 - 1）细胞培养1、2、3天，采用细胞计数试剂盒 - 8法检测细胞增殖，应用逆转录 - 聚合酶链反应（RT - PCR）和蛋白质印迹技术（Western Blotting）检测GLUT4 mRNA和蛋白的表达，并评估心肌细胞增殖与GLUT4表达之间的关系。 结果 与NC组相比，在第2天和第3天，LG、HG2、HG3组的细胞增殖（光密度值）较低，而HG1组较高（P