Comparative pharmacokinetics study of three anthraquinones in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang by high performance liquid chromatography-mass spectrometry

A specific and sensitive high performance liquid chromatography-mass spectrometric (HPLC-MS) method has been developed and validated for simultaneous determination of three anthraquinones of rhein, aloe-emodin and emodin in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang. The analytes were separated on a Kromaisl (R) C-18 column within a total running time of 12 min with a mobile phase of methanol:ammonium acetate (3 mM) (75:25, v/v). The calibration curves for all the anthraquinones showed good linearity in the measured range with correlation coefficient (r) higher than 0.9978. The precision, accuracy, recovery and stability were deemed acceptable. The method was successfully applied to the comparative pharmacokinetics study of the anthraquinones in rat plasma after oral administration of Radix et Rhei Rhizoma extract and Dahuang Fuzi Tang. (C) 2012 Elsevier B.V. All rights reserved.

建立并验证了一种专属性强、灵敏度高的高效液相色谱 - 质谱（HPLC - MS）法，用于同时测定大鼠口服大黄提取物及大黄附子汤后血浆中大黄酸、芦荟大黄素和大黄素三种蒽醌类成分。分析物在Kromaisl（R）C - 18柱上分离，总运行时间为12分钟，流动相为甲醇：醋酸铵（3 mM）（75:25，v/v）。所有蒽醌类成分的校准曲线在测定范围内呈良好的线性，相关系数（r）高于0.9978。精密度、准确度、回收率和稳定性均符合要求。该方法已成功应用于大鼠口服大黄提取物及大黄附子汤后血浆中蒽醌类成分的比较药代动力学研究。（C）2012爱思唯尔有限公司。保留所有权利。