Coaxial bioelectrospinning of P34HB/PVA microfibers biomimetic scaffolds with simultaneity cell-laden for improving bone regeneration

Poorly functioning scaffold materials and limitations in material-cell construction technology remain problems in bone tissue engineering. Electrospinning has been proven to be a suitable and available technique for production of 3D biomimetic scaffolds. To overcome these problems and retain the advantages of electrospinning, bioelectrospinning has been proposed to provide guidance by micropattern, homogeneous cell distribution, and effective nutrient utilization. Herein, we fabricated poly (3-hydroxybutyrateco-4-hydroxybutyrate)/poly (vinyl alcohol) (P34HB/PVA) with human bone mesenchymal stem cells (hBMSCs) to biomimetic core-shell microfiber/cells complex by coaxial bioelectrospinning. Characterization of the fiber materials revealed that coaxial electrospinning combined the advantages of the two materials. In vitro cellular assays showed that after bioelectrospinning, the cells and material could be simultaneously spun while maintaining cell activity. The material provided a good 3D microenvironment allowing cell adhesion, proliferation, and differentiation. Both the in vitro and in vivo tests indicated the formation of mineralized nodules in both the co-spun and co-axial groups, while the implants formed bone-like tissue after 16 weeks of implantation. This study shows that coaxial bioelectrospinning technology can be used to construct tissue-engineered bone. Our technique might be applied in the future to fabricate more complex tissue and organs. (c) 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

支架材料性能不佳以及材料 - 细胞构建技术的局限性仍然是骨组织工程中的问题。电纺丝已被证明是一种适用于生产三维仿生支架的可行技术。为了克服这些问题并保留电纺丝的优势，生物电纺丝被提出用于通过微图案、均匀的细胞分布以及有效的营养利用来提供引导。在此，我们通过同轴生物电纺丝将聚（3 - 羟基丁酸酯 - 共 - 4 - 羟基丁酸酯）/聚乙烯醇（P34HB/PVA）与人骨髓间充质干细胞（hBMSCs）制成仿生核 - 壳微纤维/细胞复合物。对纤维材料的表征表明，同轴电纺丝结合了两种材料的优势。体外细胞实验表明，生物电纺丝后，细胞和材料能够同时纺丝，同时保持细胞活性。该材料提供了良好的三维微环境，允许细胞黏附、增殖和分化。体外和体内试验均表明，共纺丝组和同轴组都形成了矿化结节，并且植入物在植入16周后形成了类骨组织。这项研究表明，同轴生物电纺丝技术可用于构建组织工程化骨。我们的技术未来可能会应用于制造更复杂的组织和器官。（c）2021作者。由爱思唯尔有限公司出版。这是一篇在CC BY - NC - ND许可（http://creativecommons.org/licenses/by - nc - nd/4.0/）下的开放获取文章。