Fluorinated Methacrylamide Chitosan Hydrogel Dressings Improve Regenerated Wound Tissue Quality in Diabetic Wound Healing.

Objective: Oxygen therapy has shown promising results for treating diabetic wounds. However, clinically used oxygen therapies are cumbersome and expensive. Thus, there is a need to develop a localized oxygenating treatment that is easy to use and inexpensive. Approach: In this study, we tested a previously developed hydrogel sheet wound dressing based on fluorinated methacrylamide chitosan (MACF) for enhanced oxygenation and compared it with a commercial sheet hydrogel dressing, AquaDerm™, and no treatment controls in a splinted transgenic diabetic mouse wound model. Results: AquaDerm exhibited poor wound closure response compared with the MACF oxygenating hydrogel sheet dressing (MACF+O2) and no treatment. Histological analysis revealed enhanced collagen synthesis and neovascularization upon MACF+O2 treatment as indicated by higher collagen content and number of blood vessels/capillaries compared with AquaDerm and no treatment. MACF+O2 also improved wound collagen fiber alignment, thus demonstrating improved skin tissue maturation. Nuclear magnetic resonance spectroscopy-based biodistribution analysis revealed that the degradation products of the MACF-based dressing did not accumulate in lung, liver, and kidney tissues of the treated animals after 14 days of treatment. Innovation: This study presents the first application of a unique oxygenating biomaterial (MACF) made into a moist hydrogel wound dressing for treating diabetic wounds. Conclusion: The results of this study confirm the benefits of this novel biomaterial approach for improving regenerated tissue structure in diabetic wound healing.

目的：氧疗在治疗糖尿病伤口方面已显示出有前景的结果。然而，临床使用的氧疗方法繁琐且昂贵。因此，有必要开发一种易于使用且价格低廉的局部充氧治疗方法。 方法：在本研究中，我们测试了一种先前开发的基于氟化甲基丙烯酰胺壳聚糖（MACF）的水凝胶片状伤口敷料的充氧效果，并将其与一种商业片状水凝胶敷料AquaDerm™以及未治疗的对照组在一种夹板固定的转基因糖尿病小鼠伤口模型中进行比较。 结果：与MACF充氧水凝胶片状敷料（MACF + O₂）和未治疗组相比，AquaDerm表现出较差的伤口闭合反应。组织学分析显示，与AquaDerm和未治疗组相比，MACF + O₂治疗后胶原蛋白合成和新血管形成增强，表现为胶原蛋白含量更高以及血管/毛细血管数量更多。MACF + O₂还改善了伤口胶原蛋白纤维排列，从而表明皮肤组织成熟度提高。基于核磁共振波谱的生物分布分析显示，在治疗14天后，基于MACF的敷料的降解产物未在接受治疗的动物的肺、肝和肾组织中积聚。 创新：本研究首次将一种独特的充氧生物材料（MACF）应用于制成湿性水凝胶伤口敷料以治疗糖尿病伤口。 结论：本研究的结果证实了这种新型生物材料方法在改善糖尿病伤口愈合中再生组织结构方面的益处。