Overexpression of ribonuclease inhibitor defines good prognosis and suppresses proliferation and metastasis in human colorectal cancer cells via PI3K/AKT pathway

We aim to evaluate the diagnostic value of ribonuclease inhibitor (RI) in colorectal cancer (CRC) and investigate the important role of RI in cell growth and metastasis of CRC.In this study, the expression of RI was evaluated in human CRC samples with different histological grade and the association between RI expression and clinicopathological parameters was investigated. Furthermore, the exogenous RI gene was introduced into human HT29 CRC cells and the effects of RI on cell proliferation and metastasis were determined in vitro. The PI3K/Akt signaling pathway and some related protein molecules were detected.RI is downregulated in CRC tissues compared to adjacent normal tissues and its expression is inversely associated with histological grade, pathological stage, and venous invasion, respectively. Multivariate analysis showed that RI expression was an independent prognostic factor for overall survival. In addition, the exogenous overexpression of RI reduced the proliferation and migration of HT29 CRC cell line in vitro by inhibiting the PI3K/Akt signaling pathway and suppressing the expression of vascular endothelial growth factor (VEGF) and upregulating phosphatase and tensin homolog (PTEN).RI represents an important predictor of progression in patients with CRC and suppresses proliferation and metastasis in CRC cells through inhibiting PI3K/AkT pathway.

我们旨在评估核糖核酸酶抑制剂（RI）在结直肠癌（CRC）中的诊断价值，并探究RI在CRC细胞生长和转移中的重要作用。在本研究中，评估了RI在不同组织学分级的人类CRC样本中的表达，并研究了RI表达与临床病理参数之间的关联。此外，将外源性RI基因导入人类HT29 CRC细胞，并在体外测定了RI对细胞增殖和转移的影响。检测了PI3K/Akt信号通路和一些相关蛋白分子。与邻近正常组织相比，RI在CRC组织中表达下调，其表达分别与组织学分级、病理分期和静脉侵犯呈负相关。多变量分析表明，RI表达是总生存期的一个独立预后因素。此外，外源性RI过表达通过抑制PI3K/Akt信号通路、抑制血管内皮生长因子（VEGF）的表达以及上调磷酸酶和张力蛋白同源物（PTEN），在体外降低了HT29 CRC细胞系的增殖和迁移。RI是CRC患者病情进展的一个重要预测指标，并通过抑制PI3K/Akt通路抑制CRC细胞的增殖和转移。