磁场条件下Fe2+ 对产聚唾液酸大肠杆菌的诱变选育

Using Escherichia coli that produces polysialic acid (PSA) as the starting strain, multiple rounds of mutagenesis and breeding of this strain were carried out using Fe2+ under magnetic field conditions to obtain a mutagenic strain with high-yield performance, and fermentation verification was carried out in a 5L fermenter. The results showed that under the condition of a 0.5T magnetic field, the optimal mutagenic mass concentration of Fe2+ was 90mg·L-1; after 5 rounds of mutagenesis and breeding, the shake flask fermentation level of PSA increased from 1.09g·L-1 to 2.24g·L-1; the fermentation level in a 5L fermenter increased from 4.79g·L-1 to 9.22g·L-1, with a 92% increase in yield.

以产聚唾液酸(polysialicacid,PSA)的大肠杆菌(Escherichiacoli)为出发菌株,在磁场条件下利用.Fe2+ 对该菌种进行多轮诱变选育,获得具有高产性能的诱变菌株,在5L发酵罐进行发酵验证.结果表明:在0.5T.磁场条件下,Fe2+ 的最佳诱变质量浓度为90mg·L-1;经过5轮诱变和选育,PSA的摇瓶发酵水平从1.09g·L-1.提高到2.24g·L-1;5L发酵罐发酵水平从4.79g·L-1提高到9.22g·L-1,产量提高了92%.