Aimed to explore the effects of probiotic Saccharomyces cerevisiae and its inactivated form on the expression of beta - defensin - 1 (sheep beta - defensin - 1, SBD - 1) in ovine rumen explants. In this study, a method for culturing ovine rumen explants was established. After co - culturing Saccharomyces cerevisiae and its inactivated form at different concentrations (104, 105, 106, 107, 108, 109 CFU·mL-1) with the explants for 24 h, the expression changes of SBD - 1 mRNA and protein in the rumen explants were detected by qPCR and ELISA methods respectively to determine the bacterial liquid concentrations at which the live bacteria and its inactivated form induced the highest expression of SBD - 1; then the live bacteria and its inactivated form at this concentration were used to treat the rumen explants for different times (2, 4, 8, 12,
旨在探索益生性酿酒酵母菌及其灭活菌对绵羊瘤胃外植体内β-防御素-1(sheep beta-defensin-1,SBD-1)表达的影响。本研究建立了绵羊瘤胃外植体培养方法,将不同浓度(104、105、106、107、108、109 CFU·mL-1)的酿酒酵母菌及其灭活菌与外植体共培养24 h后,通过qPCR和ELISA方法检测瘤胃外植体内SBD-1 mRNA和蛋白的表达变化,以分别确定活菌及其灭活菌诱导SBD-1表达最高的菌液浓度;然后用该浓度的活菌及其灭活菌对瘤胃外植体进行不同时间(2、4、8、12、