Dynamic imaging of cellular pH and redox homeostasis with a genetically encoded dual-functional biosensor, pHaROS, in yeast

Intracellular pH and redox states are critical for multiple processes and partly determine cell behavior. Here, we developed a genetically encoded dual-function probe, named p H and redox-sensitive fluorescent protein (pHaROS), for simultaneous real-time

细胞内的pH值和氧化还原状态对多个过程至关重要，并在一定程度上决定细胞的行为。在此，我们开发了一种基因编码的双功能探针，名为pH和氧化还原敏感荧光蛋白（pHaROS），用于同时进行实时（监测）。