甜茶苷的酶法制备及其对肝肠胃细胞的抑制作用

Rubusoside is a sweetener that is scarce in nature. In this experiment, a β - glucosidase from Aspergillus niger was screened out from various glycosidases to specifically hydrolyze stevioside for high - throughput preparation of rubusoside. After reacting for 12 h under the optimal conditions, the yield of rubusoside was 90.4% and the conversion rate of stevioside reached 98.8%. In order to further investigate the safety of rubusoside and expand its potential uses, the cytotoxicity of rubusoside on human gastrointestinal and liver cells was examined in the experiment, that is, the inhibitory effect of rubusoside on the growth of 3 normal cells and 11 cancer cells. The experimental results showed that at a mass concentration of 250 µg/mL, rubusoside was non - toxic to normal human gastrointestinal and liver cells; at the same mass concentration, the inhibition rate of rubusoside on human liver cancer cells BEL - 7404 was 30% of that of 5 - fluorouracil.

甜茶苷是一种自然界中含量稀少的甜味剂。本实验从多种糖苷酶中筛选出一种来自Aspergillus niger的β-葡萄糖苷酶,用以特异性水解斯替夫苷而高通量制备甜茶苷。最适条件下反应12 h后,甜茶苷的产率为90.4%,斯替夫苷的转化率达98.8%。为了进一步考察甜茶苷的安全性并拓展其潜在的用途,实验考察了甜茶苷对人胃肠道和肝脏细胞的细胞毒性,即甜茶苷对3种正常细胞以及11种癌细胞生长的抑制效果。实验结果表明,在250 µg/mL的质量浓度下,甜茶苷对正常人胃肠肝细胞无毒性;相同质量浓度下,甜茶苷对人体肝癌细胞BEL-7404的抑制率是5-氟尿嘧啶的30%。