APMP废液中半纤维素的羧甲基化及生物活性研究

Hemicellulose was extracted from the eucalyptus APMP (Alkaline Peroxide Mechanical Pulping) waste liquid by the alcohol precipitation method, and it was quantitatively and qualitatively analyzed by high-performance liquid chromatography. Using the hemicellulose extracted from the APMP waste liquid as a raw material, it was carboxymethylated and modified, and the effects of temperature (T), the amount of alkali (NaOH), and the amount of etherifying agent (ClCH2COOH) on the degree of substitution (DS) of carboxymethyl hemicellulose were explored. The properties and biological activities of hemicellulose (HC) and carboxymethyl hemicellulose (CMHC) were studied, mainly including antioxidant, hygroscopic, moisture-retention, and antibacterial properties. The results showed that the hemicellulose extracted from the APMP waste liquid had xylan as its main component; the optimal preparation process conditions for CMHC were a reaction time of 2 h, a reaction temperature of 75 °C, and a molar ratio of HC, NaOH, and ClCH2COOH of 1∶1∶2, with a maximum degree of substitution of 1.47; as determined by experiments, CMHC had better effects in terms of antioxidant, hygroscopic, moisture-retention, and antibacterial properties than HC.

采用醇沉法从桉木APMP(Alkaline Peroxide Mechanical Pulping)废液中提取半纤维素,利用高效液相色谱法对其进行定量、定性分析。以APMP废液中提取的半纤维素为原料,对其进行羧甲基化改性,并探究温度(T)、碱用量(NaOH)和醚化剂用量(ClCH2COOH)对羧甲基半纤维素的取代度(DS)的影响。对半纤维素(HC)和羧甲基半纤维素(CMHC),进行性质表征和生物活性研究,主要是抗氧化性、吸湿、保湿性和抑菌性。结果表明,APMP废液中提取的半纤维素是以木聚糖为主要成分;CMHC的最佳制备工艺条件是,反应时间2 h,反应温度75℃,HC、NaOH和ClCH2COOH的摩尔比为1∶1∶2,取代度最高为1.47;经实验测定,CMHC在抗氧化性、吸湿性、保湿性和抑菌性方面比HC有更好的效果。