辣椒碱基–抗坏血酸转运蛋白(NAT)家族基因的鉴定和表达分析

Using bioinformatics methods, 12 nucleotide - ascorbate transporter (NAT) genes were identified in pepper 'Zunla No.1' and named CaNAT01 - CaNAT12. They are unevenly distributed on 7 chromosomes, and segmental duplication and tandem duplication are the reasons for their amplification. CaNATs contain multiple transmembrane domains, and the isoelectric points mainly range from 8.39 to 10.15. Phylogenetic analysis found that NATs in plants originated from group D genes and then differentiated. There are 4 stable subgroups in dicotyledonous plants. The nucleotide differences among group C genes are the smallest and they are the most conserved during evolution. CaNATs are differently distributed in these 4 subgroups. The genes of the pepper NAT family have obvious tissue expression differences, and some genes play important regulatory roles in the roots, stems and fruit development process. Through qRT - PCR analysis under low temperature, high temperature, drought and salt stress, it was found that CaNATs have different degrees of response and a relatively strong response to low temperature and high temperature.

利用生物信息学手段,在辣椒‘遵辣1号’中鉴定到12个碱基–抗坏血酸转运蛋白(NAT)基因,命名为CaNAT01 ~ CaNAT12,它们不均等的分布在7条染色体上,片段复制和串联复制是其扩增的原因。CaNATs包含多个跨膜结构域,等电点主要集中在8.39 ~ 10.15。系统进化分析发现植物中的NAT起源于D亚组基因,后经过分化,在双子叶植物中稳定存在4个亚组,C亚组基因间的核苷酸差异最小,在进化过程中最保守,CaNATs在这4个亚组均有不同分布。辣椒NAT家族基因具有明显的组织表达差异性,部分基因在根、茎以及果实发育过程中具有重要的调控作用。在低温、高温、干旱和盐胁迫下通过qRT-PCR分析发现CaNATs具有不同程度的响应,对低温和高温具有较强的响应。