Persistent cognitive deficits and neuroinflammation in a rat model of cardiopulmonary bypass.

Legend for Graphical Abstract. A time line of the study is shown. Surgery is performed on Day 0 of the experiment and the CPB circuit is illustrated (adapted from Grocott et al.). At 5½ months post-CPB, food restriction is initiated. Starting 6 months post-CPB, behavioral testing is performed using an 8-arm maze. Animals are euthanized at the end of behavioral testing via perfusion fixation, and cellular analyses on tissue sections are performed using histological and immunohistochemical techniques. Cognitive impairment and increased neuroinflammation were key findings in the CPB group.

图形摘要说明。展示了该研究的时间线。在实验的第0天进行手术，并对体外循环（CPB）回路进行了图示（改编自格罗科特等人的研究）。在体外循环后5个半月，开始限制食物。从体外循环后6个月开始，使用八臂迷宫进行行为测试。在行为测试结束时，通过灌注固定对动物实施安乐死，并使用组织学和免疫组织化学技术对组织切片进行细胞分析。认知障碍和神经炎症增加是体外循环组的关键发现。