关于改良酶法人源动脉脱细胞基质的制备方法研究

Congenital biliary atresia is the result of poor embryonic development. Just imagine that if one's own tissues are used to repair congenital and acquired defects, not only the immune rejection reaction is excluded, but it is also economical and inexpensive. The methods of decellularization can be roughly divided into three types: mechanical or physical methods, chemical reagent methods and enzymatic methods. After experimental selection, enzymatic digestion and detergent methods are now used for the preparation of decellularized matrix. Currently, most cases of congenital biliary atresia require surgical treatment. The preparation of autologous arterial decellularized matrix brings new opportunities for the treatment of this disease. Whether the autologous artery can be used to repair the biliary stricture site has become a new research direction and at the same time lays the foundation for the subsequent transplantation of porcine-induced biliary epithelial cells.

先天性胆道闭锁是胚胎发育不良的结果。试想一下，如果用自身的组织去修复先天以及后天缺损，既排除了免疫排斥反应， 又经济廉价。脱细胞的方法大概可分为3种类型：机械或物理的方法、化学试剂的方法和酶解的方法。经过试验选择，现采用酶消化， 去污剂方法进行脱细胞基质的制备。现在，绝大部分的先天性胆道闭锁需要外科手术治疗，自体动脉脱细胞基质的制备给这一疾病的 治疗带来新的契机，能否用自体动脉修复胆道狭窄部位成为新的研究方向，同时为后续猪诱导型胆管上皮细胞的移植奠定了基础。