PBAT hollow porous microfibers prepared via electrospinning and their functionalization for potential peptide release

Although microfiber (MF) loaded polypeptides have been widely studied in the field of medicine, load control and slow release remain significant challenges. Herein, polybutylene adipate terephthalate (PBAT) hollow porous MFs (HPMFs) were prepared by improving and regulating the groups and struc-tures of the MFs to address these challenges. The capacity of the HPMFs for the loading of polypeptide can be improved. Measurements involving the use of X-ray photoelectron, energy-dispersive, and ultra-violet (UV) spectroscopies in conjunction with scanning electron microscopy measurements showed that the amount of polypeptide (arginylglycylaspartic acid, RGD) loaded on the HPMFs was significantly higher than that loaded on the MFs. Animal cell experiments revealed that PBAT grafted MAH has good bioactivity. By loading RGD onto HPMFs and MFs, RGD@HPMFs and RGD@MFs were obtained. A study on HeLa and A549 cancer cells showed that the inhibition rates of RGD@HPMFs were higher than that of RGD@MFs by 14.1% and 6.9%, respectively. The results obtained herein show that HPMF scaffold prepa-ration by improving the material groups and regulating the structure of MFs can address the challenges associated with control of the load and sustained release of polypeptides and other drugs. (c) 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

尽管载有多肽的微纤维（MF）在医学领域已被广泛研究，但负载控制和缓释仍然是重大挑战。在此，通过改进和调控微纤维的基团和结构制备了聚己二酸/对苯二甲酸丁二醇酯（PBAT）中空多孔微纤维（HPMFs）以应对这些挑战。HPMFs对多肽的负载能力能够得到提高。结合使用X射线光电子能谱、能量色散谱和紫外（UV）光谱以及扫描电子显微镜测量表明，HPMFs上负载的多肽（精氨酰 - 甘氨酰 - 天冬氨酸，RGD）量明显高于MFs上负载的量。动物细胞实验显示PBAT接枝马来酸酐（MAH）具有良好的生物活性。通过将RGD负载到HPMFs和MFs上，得到了RGD@HPMFs和RGD@MFs。对HeLa和A549癌细胞的研究表明，RGD@HPMFs的抑制率分别比RGD@MFs高14.1%和6.9%。本文所得结果表明，通过改进材料基团和调控MFs的结构来制备HPMF支架能够解决与多肽及其他药物的负载控制和缓释相关的挑战。（c）2021作者。由爱思唯尔有限公司出版。这是一篇在CC BY - NC - ND许可（http://creativecommons.org/licenses/by - nc - nd/4.0/）下的开放获取文章。