Chromatin assembly factor - 1 (CAF - 1) is a histone chaperone composed of three subunits: p150, p60 and p48. The main function of CAF - 1 is to interact with proliferating cell nuclear antigen (PCNA) during DNA replication and is responsible for recruiting histones H3 and H4 to deposit on newly synthesized DNA to promote nucleosome assembly. An increasing number of studies have analyzed the structure and biological functions of the CAF - 1 complex and found that CAF - 1 plays an important role in the process of somatic cell reprogramming. Therefore, this article focuses on the structure, biological functions of CAF - 1 and its role in somatic cell reprogramming.
染色质组装因子1(chromatin assembly factor-1,CAF-1),是由p150、p60、p48三个亚单位组成的组蛋白伴侣。CAF-1主要功能是在DNA复制中与增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)相互作用,负责募集组蛋白H3、H4沉积在新合成的DNA上以促进核小体装配。越来越多的研究解析CAF-1复合体的结构及其生物学功能,并发现CAF-1在体细胞重编程过程中发挥重要作用。因此,本文重点介绍了CAF-1的结构、生物学功能和在体细胞重编程中的作用。