链格孢菌毒素胁迫下的棘孢木霉转录组构建及生防基因差异表达

In order to explore the functional regulatory network of Trichoderma under the stress of Alternaria alternata toxin, samples were taken for sequencing at different times of toxin stress, and the transcriptome of Trichoderma asperellum CGMCC11653 under toxin stress was established. The results showed that the alignment efficiency of Reads in each sample with the reference genome was 95.63% - 97.50%, and the assembly of the selected reference genome could meet the requirements of information analysis. After toxin stress, there were 3866 differentially expressed genes in Trichoderma; GO classification found that the transcripts at 6, 12, and 48 h were enriched with 292, 551, and 3023 genes in the three categories of biological process, molecular function, and cellular component, respectively. The analysis of defense genes regulated by Trichoderma at different time points found that the changes of various disease-resistant genes were not obvious before 12 h of toxin stress, but after 48 h of stress, Trichoderma regulated most of the disease-resistant related genes to respond, including ABC transporter ABC1 (3.44 times), heat shock protein gene Hsp1 (2.41 times), glucan gene Glucan16 (2.91 times), etc., indicating that Trichoderma can precisely regulate the stress of pathogenic fungal toxins through related resistance genes.

为了探究链格孢菌（Alternariaalternata）毒素胁迫条件下木霉的功能调控网路，在毒素胁迫的不同时间取样测序，建立了毒素胁迫下棘孢木霉（Trichoderma asperellum）CGMCC11653的转录组。结果表明：各样品的Reads 与参考基因组的比对效率为95.63％ ～97.50％，所选参考基因组组装能够满足信息分析的需求。毒素胁迫后，木霉中有3866个变化的差异表达基因；Go分类发现6、12、48h 的转录本在生物学过程、分子功能和细胞组分3个类别中分别富集了292、551、3023个基因。对在不同时间点下木霉调控的防卫基因分析发现，毒素胁迫12h前各类抗病基因变化并不明显，但胁迫48ｈ 后木霉调控了大部分抗病相关基因做出反应，包括ABC转运蛋白ABC1（3.44倍）、热激蛋白基因Hsp1（2.41倍）葡聚糖基因Glucan16（2.91倍）等，表明木霉可以通过相关抗性基因病原真菌毒素胁迫进行精准调控。