Molecular phylogeny of the family Dicroglossidae (Amphibia: Anura) inferred from complete mitochondrial genomes

The fork-tongued frogs, members of the amphibian Order Anura, belong to the family Dicroglossidae and are one of the most diverse groups of Anuran frogs; however, their taxonomy and phylogeny remain controversial. In the present study, sixteen dicroglossine mitochondrial genomes representing nine dicroglossine genera and 23 other neobatrachian taxa, were used to reconstruct the phylogenetic relationships of the family Dicroglossidae using different partitioned maximum likelihood and partitioned Bayesian inference methods at both the nucleotide and amino acid levels. The sampled fork-tongued frogs form a strongly supported monophyletic group that is the sister taxon to another well-supported Glade that includes representatives of the families Ranidae, Rhacophoridae, and Mantellidae. The monophyly of the subfamily Occidozyginae and Dicroglossinae was revealed with strong supports, and two major clades were supported within Dicroglossinae. The sister-group relationship between the genera Limnonectes and the tribe Paini was supported. In addition, a sister-group relationships between Fejervarya and Euphlyctis + Hoplobatrachus, between Quasipaa and Yerana, and between Feirana and Nanorana are well supported. Estimates of divergence times revealed the divergence of Dicroglossidae during the Late Upper Cretaceous to the Early Eocene, and diversification of the major dicroglossine genera from the Early Eocene to the Middle Miocene. (C) 2017 Elsevier Ltd. All rights reserved.

叉舌蛙是两栖纲无尾目成员，属于叉舌蛙科，是无尾目蛙类中最多样化的类群之一；然而，它们的分类学和系统发育仍存在争议。在本研究中，选取了代表9个叉舌蛙属的16个叉舌蛙线粒体基因组以及23个其他新蛙亚目分类单元，在核苷酸和氨基酸水平上，使用不同的分区最大似然法和分区贝叶斯推断方法来重建叉舌蛙科的系统发育关系。所选取的叉舌蛙形成了一个具有强支持的单系群，它是另一个具有强支持的分支的姊妹分类单元，该分支包括蛙科、树蛙科和曼蛙科的代表物种。姬蛙亚科和叉舌蛙亚科的单系性得到了有力支持，并且在叉舌蛙亚科内有两个主要分支也得到了支持。林蛙属与湍蛙族之间的姊妹群关系得到了支持。此外，泽蛙属与狭口蛙属 + 虎纹蛙属之间、棘蛙属与叶氏蛙属之间以及棘胸蛙属与倭蛙属之间的姊妹群关系也得到了有力支持。分歧时间的估算表明，叉舌蛙科在上白垩世晚期至始新世早期发生分歧，主要的叉舌蛙属在始新世早期至中新世中期发生多样化。（C）2017爱思唯尔有限公司。保留所有权利。