A Mouse Model for Chronic Pancreatitis via Bile Duct TNBS Infusion.

Chronic pancreatitis (CP) is a complex disease involving pancreatic inflammation and fibrosis, glandular atrophy, abdominal pain and other symptoms. Several rodent models have been developed to study CP, of which the bile duct 2,4,6 -trinitrobenzene sulfonic acid (TNBS) infusion model replicates the features of neuropathic pain seen in CP. However, bile duct drug infusion in mice is technically challenging. This protocol demonstrates the procedure of bile duct TNBS infusion for generation of a CP mouse model. TNBS was infused into the pancreas through the ampulla of Vater in the duodenum. This protocol optimized drug volume, surgical techniques, and drug handling during the procedure. TNBS-treated mice showed features of CP as reflected by bodyweight and pancreas weight reductions, changes in pain-associated behaviors, and abnormal pancreatic morphology. With these improvements, mortality associated with TNBS injection was minimal. This procedure is not only critical in generating pancreatic disease models but is also useful in local pancreatic drug delivery.

慢性胰腺炎（CP）是一种复杂的疾病，涉及胰腺炎症和纤维化、腺体萎缩、腹痛及其他症状。已经开发了几种啮齿动物模型来研究慢性胰腺炎，其中胆管2,4,6 - 三硝基苯磺酸（TNBS）灌注模型可模拟慢性胰腺炎中出现的神经性疼痛特征。然而，在小鼠中进行胆管药物灌注在技术上具有挑战性。本方案展示了通过胆管TNBS灌注构建慢性胰腺炎小鼠模型的操作过程。TNBS通过十二指肠的瓦特氏壶腹注入胰腺。该方案优化了操作过程中的药物剂量、手术技术以及药物处理。经TNBS处理的小鼠表现出慢性胰腺炎的特征，具体体现为体重和胰腺重量减轻、疼痛相关行为改变以及胰腺形态异常。通过这些改进，TNBS注射相关的死亡率极低。这一操作过程不仅对构建胰腺疾病模型至关重要，而且对局部胰腺给药也很有用。