The copy number of exogenous genes in transgenic plants is an important factor affecting their genetic stability and expression level. This study aims to measure and analyze the copy number of exogenous genes and the lipoxygenase activity in the seeds of transgenic wheat (Triticum aestivum) with an RNAi sequence of an exogenous seed lipoxygenase gene (RNAi sequence of lipoxygenase gene, Loxi), in order to obtain new transgenic wheat germplasm with significantly reduced seed lipoxygenase activity and stable inheritance of exogenous Loxi. Using TaqMan real-time quantitative PCR technology, with the major single-copy gene Pinb (puroindoline b) that controls grain hardness in wheat as an internal reference gene, for...
转基因植物中外源基因拷贝数是影响其遗传稳定性及自身表达水平的重要因素。本研究旨在对转外源种子脂氧合酶基因的RNAi序列(RNAi sequence of lipoxygenase gene,Loxi)的小麦(Triticum aestivum)转基因株系中外源基因拷贝数及其种子脂氧合酶活性进行测定分析,以期获得种子脂氧合酶活性显著降低而且外源Loxi能稳定遗传的转基因小麦新种质。采用TaqMan实时定量PCR技术,以小麦内源控制籽粒硬度的主效单拷贝基因Pinb(puroindoline b)为内参基因,对