Introduction to protein crystallization

Biological macromolecules can be crystallized by a variety of techniques, and using a wide range of reagents which produce supersaturated mother liquors. These may, in turn, be applied under different physical conditions such as temperature. The fundamental approaches to devising successful crystallization conditions and the factors that influence them are summarized here. For the novice, it is hoped that this brief review might serve as a useful introduction and a stepping-stone to a successful X-ray strucutre determination. In addition, it may provide a framework in which to place the articles that follow. (C) 2004 Elsevier Inc. All rights reserved.

生物大分子可以通过多种技术结晶，并使用各种各样能产生过饱和母液的试剂。这些技术和试剂又可以在不同的物理条件（如温度）下应用。这里总结了设计成功结晶条件的基本方法以及影响这些条件的因素。希望对于新手来说，这篇简短的综述可以作为有用的介绍以及通向成功的X射线结构测定的垫脚石。此外，它还可能提供一个框架，以便放置后续的文章。（C）2004爱思唯尔公司。保留所有权利。