Antiviral effect of ovotransferrin in mouse peritoneal macrophages by up-regulating type I interferon expression

Ovotransferrin (OVT) is one of the major proteins in avian albumen that exerts diverse biological activities. This study is aimed to investigate the antiviral effect and mechanism of OVT from the avian egg. Effects of OVT on vesicular stomatitis virus (VSV)-infected mouse peritoneal macrophage were detected by realtime quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and western blot. In OVT-pretreated mouse peritoneal macrophages, results showed that replication of VSV was significantly suppressed and production of type I interferon (IFN) was increased. OVT down-regulated ring finger protein 125 (RNF125) production and up-regulated retinoic-acid-inducible gene I (RIG-I) expression by the nuclear factor-κB signalling pathway, resulting in the enhancement of the production of type I IFN. It demonstrated that the antiviral effect of OVT on innate immunity is enhanced by promoting type I IFN expression, indicating its potential as a functional food ingredient.

卵转铁蛋白（OVT）是禽类蛋清中的主要蛋白质之一，具有多种生物活性。本研究旨在探讨禽类卵中卵转铁蛋白的抗病毒作用及其机制。通过实时定量聚合酶链反应、酶联免疫吸附测定和蛋白质印迹法检测卵转铁蛋白对水疱性口炎病毒（VSV）感染的小鼠腹腔巨噬细胞的影响。在经卵转铁蛋白预处理的小鼠腹腔巨噬细胞中，结果显示VSV的复制受到显著抑制，I型干扰素（IFN）的产生增加。卵转铁蛋白通过核因子 - κB信号通路下调无名指蛋白125（RNF125）的产生并上调维甲酸诱导基因I（RIG - I）的表达，从而导致I型干扰素产生增加。这表明卵转铁蛋白通过促进I型干扰素表达增强了对先天免疫的抗病毒作用，显示其作为一种功能性食品成分的潜力。