Beclinl基因沉默对胰腺癌MiaPaCa-2细胞生长的影响

The Beclin1 gene is homologous to the yeast autophagy gene Atg6 and is an important gene involved in the formation of autophagosomes in mammals and the regulation of the autophagy process. Defects in the Beclin1 autophagy gene may cause tumor cells to evade autophagic death [1], while stable transfection of Beclin1 can promote the autophagy activity of cells and reduce their tumorigenic ability [2]. This study observed the effects of silencing the expression of the Beclin1 gene in pancreatic cancer MiaPaCa - 2 cells on their proliferation, apoptosis and cell cycle distribution, providing new ideas for the gene therapy of pancreatic cancer. I. Materials and Methods 1. Construction of siRNA expression vectors targeting Beclin1: According to the principle of siRNA design, three insertions targeting Beclin1 were constructed by Shanghai GenePharma Co., Ltd.

Beclin1基因与酵母自噬基因Atg 6同源,是参与哺乳动物自噬体形成,调控细胞自噬过程的重要基因.Beclin1自噬基因的缺陷可能导致肿瘤细胞逃避自噬性死亡[1],而稳定转染Beclin1后可促进细胞的自噬活性,并降低了其成瘤能力[2].本研究观察沉默胰腺癌MiaPaCa-2细胞的Beclin1基因表达后对其增殖、凋亡及细胞周期分布的影响,为胰腺癌的基因治疗提供新的思路.一、材料与方法1.靶向Beclin1的siRNA表达载体构建:根据siRNA设计原理,由上海吉玛制药技术有限公司构建3个插入靶向Be