Skin graft take rates, granulation, and epithelialization: dependence on myeloid cell hypoxia-inducible factor 1alpha.

Production of the alpha subunit of Hypoxia Inducible Factor (HIF-1α) is increased in healing wounds, which stimulates expression of Vascular Endothelial Growth Factor (VEGF) to promote angiogenesis. Therefore, take rate of skin grafts may be closely associated with the presence or absence of HIF-1α production in the recipient bed. We compared the take rates of skin grafts between myeloid selective HIF-1α knock-out (KO) and wild-type (WT) mice. Percentage of healthy graft areas obtained by planimetry and scores for epithelialization and granulation tissue formation obtained by histopathological analysis were compared in twelve KO and twelve WT mice following skin grafting. Graft take rate was significantly impaired in the KO group, while epithelialization or granulation tissue formation scores did not reveal any significant differences. HIF-1α in myeloid cells may be an important molecule for re-vascularization of avascular tissues such as skin grafts, probably due to its stimulating effect on angiogenesis.

在愈合的伤口中，缺氧诱导因子（HIF - 1α）的α亚基的产生增加，这会刺激血管内皮生长因子（VEGF）的表达以促进血管生成。因此，皮肤移植物的成活率可能与受区是否产生HIF - 1α密切相关。我们比较了骨髓选择性HIF - 1α敲除（KO）小鼠和野生型（WT）小鼠皮肤移植物的成活率。 对12只KO小鼠和12只WT小鼠在皮肤移植后，通过平面测量法获得的健康移植物面积百分比以及通过组织病理学分析获得的上皮形成和肉芽组织形成评分进行了比较。 KO组的移植物成活率显著降低，而上皮形成或肉芽组织形成评分没有显示出任何显著差异。 骨髓细胞中的HIF - 1α可能是皮肤移植物等无血管组织再血管化的重要分子，这可能是由于其对血管生成的刺激作用。